Project
# | Title | Team Members | TA | Documents | Sponsor |
---|---|---|---|---|---|
3 | Portable PCR Machine |
Hershel Rege Nathan Franczyk Yihao Yang |
proposal0.pdf |
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-Hershel Dave-Rege davereg2 -Nathan Franczyk frnczyk2 -Yihao Yang yyang150 Problem Statement: Conventional methods for PCR (polymerase chain reaction) are time-consuming, and costly with no room for mobility in the design of current machines. Solution: Develop a self-contained, portable PCR machine. Scope of Project: Developing an entire PCR system with fully automatic functionality is out of scope for a semester project. Therefore, we propose to limit the scope to developing a portable machine (max 25 pounds) that autonomously completes the thermal cycling of 2 samples of DNA, with real-time thermal sensor measurements. System Description: Our idea is to develop an all-in-one portable system that contains all the same elements as a traditional PCR machine, but on a miniaturized scale. The different blocks within the system include: -Infrared LEDs to heat up the samples and miniature fans to cool down the samples -Thermocouples connected to a high resolution analog-to-digital converter to get accurate real-time temperature measurements -Microcontroller interface to automate the thermal cycling -USB interface to computer for display of real-time measurements -Powered by rechargeable battery -An enclosure to properly house the samples and all associated components (battery, PCBs) Test of Functionality: The system’s success can be verified by affirming that the system can operate for 30 thermal cycles continuously without losing power, and consistently hits the temperature benchmarks of 75 to 95 degrees Celsius for each cycle. The system should be able to achieve heating rates of 6 degrees/second and cooling rates of 3 degrees/second, and should not weigh over 25 pounds. |