|52||Carney Confocal Microscopy
Sung Hun Kim
Ye Hyun Kim
|Problem: Confocal Microscopy is an optical imaging technique for obtaining high resolution of mostly used in biological science. Confocal microscope uses point illumination method and discards any other stray light. Conventionally, to measure different points of sample, we had to change the angle of the light, so that it can measure different points of sample. This method may cause inaccuracy in imaging the object, since if the angle is slightly off, the light may not transmit through the pinhole, and cannot detect the image properly. In an attempt to resolve these drawbacks, we will be using 4 piezos to control the glass side. Using this method will not require changing the angle of the light anymore.
Solution: Below the glass slides, we will insert 4 piezos to control the Z-axis. In this way, we can control the height, and by controlling individual piezos, we can also tilt the glass slides. In order to function this way, we need to use a microcontroller unit, and control the piezeos. Afterwards, we need to program the device using C-language, so that the glass slide can move in Z-axis. Before implementing into the confocal microscope, we will test whether the piezos perform properly by verifying the positions. Since we are working in a nanometer scale, we need a light source and a photo-detector, and measure the speed taken. In this way, we can measure the distance moved, and confirm whether the glass slide has moved as we have expected.
Challenge: This project requires measuring in nanometer scale, which is not really visible to human eyes. Consequently, we would need to work on using light source and photo-detector, which requires the usage of programming. Although we have not explicitly learned to program the microcontroller unit, we can analyze the datasheet. Also, we would need a very sensitive photo-detector to measure the speed taken.
Ye Hyun Kim (ykim102)
Sung Hun Kim (skim113)
Hyunjae Cho (cho135)