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Dr. Jenny Amos
Office hours: open door
Bioengineering 498 JRA. Bioreactor lab is a course that provides hands on experiences relating to tissue engineering and bioreactor design. Students will perform experiments that simulate a graduate experience by designing systems, running the experiments, performing analysis and reporting findings. Students will be given assignments relating to current literature and state of the art in the area of tissue engineering to be explored. Projects will be assigned for small teams, 2-3 students, to divide up workload needed for culture. Students will also keep a laboratory notebook and submit a report after completion of the project, including a presentation to the class.
Class meets M 9:00-9:50 am in 3211 DCL.
Lab will meet in 3116 DCL 3:00-6:00pm Mondays and Wednesdays.
- (b) an ability to design and conduct experiments, as well as to analyze and interpret data
- (c) an ability to design a system, component, or process to meet desired needs within realistic constraints such as economic, environmental, social, political, ethical, health and safety, manufacturability, and sustainability
- (d) an ability to function on multidisciplinary teams
- (e) an ability to identify, formulate, and solve engineering problems
- (f) an understanding of professional and ethical responsibility
None, all lab protocls will be provided.
Tissue Engineering, W. Mark Saltzman, Oxford University Press, 2004.
Biomaterials Science: An Introduction to Materials in Medicine, Second Edition, Buddy Ratner et. al., Elsevier Academic Press, 2004.
Cell and Tissue Reaction Engineering, Regine Eibl, Dieter Eibl, Ralf Pörtner, et. al., Springer, 2008. E-book through UIUC libraries
- Lab Practicals 30%
- Homeworks and Written Reports 40%
- In lab quizzes 20%
- Attendance 10%
- Total 100%
Lab quizzes: Approximately weekly; will build upon topics covered in lecture.
Note: Students may discuss reports, but should write up reports independently.
Lab practicals to test skills leanred may be given at any time.
|1||8/25||Overview & Lab 1: Biocompatibility||Cell review||8/27||Cell review|
||no class||9/3||Lab 1: Culture cells and Practical|
|3||9/8||No class - Frontiers||Lab 1: start culture in 6 well plate||9/10||Lab 1: add samples|
|3||9/15||Contamination||Lab 1: Observe||9/17||Lab 1: Fix, stain|
|4||9/22||Lab 2: Blood type||Lab 2: DNA isolation, PCR||9/24||Lab 2: Digests|
|6||9/29||Lab 3: Collagen Gel Project - Sensors||Lab 2: Electrophoresis||10/1||Lab 2: Carry-over|
|7||10/6||Lab 3: Collagen Gel Project||Lab 3: Sensor design||10/8||Lab 3: sensor design|
|8||10/13||Amos Out||Lab 3: sensor design and thaw cells||10/15||Lab 3: Culture cells|
|9||10/20||Imaging and Ethics in Research||Lab 3: collagen gel start||10/22||Lab 3: collect data, release gel|
|10||10/27||Lab 3: Microenvironments||lab 3: collect data, stain||10/29||Lab 3: Image|
|11||11/3||Lab 4: Hydrogels||Lab 4: Collect data, fix||11/5||
Lab 3: Stain and image and
Lab 4: Prepare stock solutions for hydrogels
|12||11/10||Lab 4: Cells on stiff materials||Lab 4: Coverslip prep and gel prep||11/12||Lab 4: Substracte functionalization, stamping, seeding|
|13||11/17||AFM Overview||Lab 4: Fix cells||11/19||Lab 4: Stain cells|
||Thanksgiving Break||Thanksgiving Break||
|14||12/1||AFM II, ICES||AFM||12/3||AFM|
|16||12/8||no lec!||Clean up||12/10||Clean up|
*You are assigned lab time in which to finish your tasks, for some labs, you may finish your work before time is up, for others you may need to work additional hours outside of lab time. You are expected to work as much as needed to perform at or above my expectations.