Course Website
Students should check this course website regularly.
Instructor
Dr. Jenny Amos
jamos@illinois.edu
1270 DCL
217-333-4212
Office hours: open door
Description
Bioengineering 498 JRA. Bioreactor lab is a course that provides hands on experiences relating to tissue engineering and bioreactor design. Students will perform experiments that simulate a graduate experience by designing systems, running the experiments, performing analysis and reporting findings. Key areas of emphasis will be parametric design of experiments using bioreactors (control and systems design). Students will be given assignments relating to current literature and state of the art in the area of tissue engineering to be explored. Projects will be assigned for small teams, 2-3 students, to divide up workload needed for culture. Students will also keep a laboratory notebook and submit a report after completion of the project, including a presentation to the class.
Class meets M 9:00-9:50 am in DCL.
Lab will meet in 3116 DCL or L512 DCL.
Expected Outcomes
- (b) an ability to design and conduct experiments, as well as to analyze and interpret data
- (c) an ability to design a system, component, or process to meet desired needs within realistic constraints such as economic, environmental, social, political, ethical, health and safety, manufacturability, and sustainability
- (d) an ability to function on multidisciplinary teams
- (e) an ability to identify, formulate, and solve engineering problems
- (f) an understanding of professional and ethical responsibility
Required Textbook
BIOE 498 Bioreactor Lab Manual, Jenny Amos, 2012.
Reference texts
Tissue Engineering, W. Mark Saltzman, Oxford University Press, 2004.
Biomaterials Science: An Introduction to Materials in Medicine, Second Edition, Buddy Ratner et. al., Elsevier Academic Press, 2004.
Cell and Tissue Reaction Engineering, Regine Eibl, Dieter Eibl, Ralf Pörtner, et. al., Springer, 2008. E-book through UIUC libraries
Grading
- Lab Practicals 20%
- Homeworks and Written Reports 40%
- Midterm 20%
- Final 20%
- Total 100%
Grading Scale
- A+>97%>A>93%>A-
- B+>87%>A>83%>B-
- C+>77%>C>73%>C-
- D+>67%>D>63%>D-
- F<60%
Homework, Deliverables and Projects: Approximately weekly; will build upon topics covered in lecture.
Note: Students may discuss homework problems, but should write up solutions independently and show all work. Projects are assumed to be group work and only 1 copy needs to be sumbitted per team. Lab practicals to test skills leanred may be given at any time.
Schedule
| Week | First Date | Lecture Topic | Lab Day 1 | Lab Day 2 |
| 1 | 8/27/2012 | Overview, Lab 1A | make media, thaw 3T3 cells, change media on ORL UVA cells | subculture of 3T3 cells, cell count, begin differentiation of ORL UVAs |
| 2 | 9/3/2012 | no lec | Labor Day | make agar, continue differentiation of ORL UVAs |
| 3 | 9/10/2012 | AFM I | prepare cell/agar mixture, continue differentiation of ORL UVAs | Continue cell culture, pick samples for testing |
| 4 | 9/17/2012 | AFM II | plate ORL UVAs in well dishes (add samples 1-2 hours after plating) | read 48 hour results, perform AP, wash materials |
| 5 | 9/24/2012 | AFM quiz | AFM Training with instructor as a group | AFM time for groups 1-2 |
| 6 | 10/1/2012 | Microscopy | AFM time for groups 3-4 | AFM time for groups 1-2 |
| 7 | 10/8/2012 | Heart TE | AFM time for groups 3-4, Obtain P19 cells from TA, build heart bioreactor | Free AFM Time, Sterilize bioreactor, feed cells |
| 8 | 10/15/2012 | Heart, Ligament TE | seed cells into reactor, decellularize | Feed cells in Bioreactor, decellularize |
| 9 | 10/22/2012 | Ligament TE | Start shock protocol, build ligament reactor, decellularize | observe cells, build ligament reactor, decellularize |
| 10 | 10/29/2012 | Review for Midterm and Ligament TE | observe cells, decellularize | observe cells, fix cells, perm primary, deccelluluarize |
| 11 | 11/5/2012 | midterm | stain cells, phalloidin, dapi (both heart and ligament), seed ligament | stretch ligament |
| 12 | 11/12/2012 | review midterm, vascular lab | change media in bioreactor, planning for project | end stretch, start RNA, seed scaffolds for project lab |
| 13 | 11/19/2012 | No class | TA and Instructor will Maintain cells | TA and Instructor will Maintain cells |
| 14 | 11/26/2012 | Vascular TE, Project Updates | RNA-> cDNA, install scaffolds in reactors | DNA-> PCR, change media in reactors |
| 15 | 12/3/2012 | No class | uninstall scaffolds, fix, perm, primary | Secondary before lab, phal, dapi |
| 16 | 12/10/2012 | Talk about Final and wrap-up class | Image cells | Clean up! |
| Finals | Final Exam Due | |||
*You are assigned lab time in which to finish your tasks, for some labs, you may finish your work before time is up, for others you may need to work additional hours outside of lab time. You are expected to work as much as needed to perform at or above my expectations.